The COP9 signalosome (CSN) is a multifunctional protein complex that regulates protein degradation through removal of the ubiquitin-like modifier Nedd8 from cullin-based E3 ubiquitin ligases. The impact of CSN loss of function in vitro and in vivo was analyzed in different periods of neuronal development by conditional knockdown of its catalytic subunit CSN5, via the Cre-loxP system. Loss of CSN5 was associated with altered neuronal migratory behavior during cortex formation in utero and reduction of dendritic length and arborization, while not affecting cognitive performance in the adult stage. It is suggested that cullin-based regulatory mechanisms may exhibit developmental stage-dependent activity patterns in the brain.      «

The COP9 signalosome (CSN) is a multifunctional protein complex that regulates protein degradation through removal of the ubiquitin-like modifier Nedd8 from cullin-based E3 ubiquitin ligases. The impact of CSN loss of function in vitro and in vivo was analyzed in different periods of neuronal development by conditional knockdown of its catalytic subunit CSN5, via the Cre-loxP system. Loss of CSN5 was associated with altered neuronal migratory behavior during cortex formation in utero and reducti...     »