Migration of plasmodia of Physarum polycephalum on plain agar and SDM agar

The plasmodia were imaged with a Zeiss Axio Zoom V.16 microscope equipped with a custom-made Pecon stage-top incubation system, a Hamamatsu ORCA-Flash 4.0 digital camera and a Zeiss PlanApo Z 0.5x objective. A green filter (550/50 nm) was placed over the transmission light source of the microscope. Zeiss Zen 3.2 (blue edition) software was used for imaging. A tiled image composed of 16 tiles was acquired using the Zen Tiles tool. All tiles were converted into 8-bit TIFF files using Zeiss Zen 3.2 and their backgrounds were removed with a rolling ball algorithm. The MIST stitching algorithm was used to assemble the tiles into a single image.     «

The plasmodia were imaged with a Zeiss Axio Zoom V.16 microscope equipped with a custom-made Pecon stage-top incubation system, a Hamamatsu ORCA-Flash 4.0 digital camera and a Zeiss PlanApo Z 0.5x objective. A green filter (550/50 nm) was placed over the transmission light source of the microscope. Zeiss Zen 3.2 (blue edition) software was used for imaging. A tiled image composed of 16 tiles was acquired using the Zen Tiles tool. All tiles were converted into 8-bit TIFF files using Zeiss Zen 3.2...     »