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- Original title:
- Phänotypische und funktionelle Auswirkungen des Knockdowns und Regulation der Genexpression von ADAMTS7 in humanen glatten Gefäßmuskelzellen
- Translated title:
- Phenotypic and functional effects of ADAMTS7 knockdown and regulation of ADAMTS7 expression in human vascular smooth muscle cells
- Author:
- Schindler, Patrick Michael
- Year:
- 2020
- Document type:
- Dissertation
- Faculty/School:
- Fakultät für Medizin
- Advisor:
- Schunkert, Heribert (Prof. Dr.)
- Referee:
- Schunkert, Heribert (Prof. Dr.); Mägdefessel, Lars (Prof. Dr.)
- Language:
- de
- Subject group:
- MED Medizin
- Abstract:
- Das A disintegrin and metalloproteinase with thrombospondin motifs 7- (ADAMTS7) Gen ist mit der Koronaren Herzkrankheit assoziiert. Diese Arbeit umfasst eine funktionelle und phänotypische Charakterisierung ADAMTS-7-defizienter humaner glatter Gefäßmuskelzellen, Untersuchungen zur ADAMTS7-Expressionsregulation und eine Evaluation spezifischer Antikörper. ADAMTS-7-Defizienz verminderte die Zellmigration unter proatherogenen Bedingungen nicht. Oxidiertes Low-density lipoprotein bewirkte eine Reduktion der ADAMTS7-Expression, welche durch Knockdown des Oxidized low-density lipoprotein receptor 1 teilweise reversibel war.
- Translated abstract:
- The A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) gene is associated with coronary artery disease. This work comprises a functional and phenotypical characterization of ADAMTS-7 deficient human vascular smooth muscle cells, analyses of ADAMTS7 gene expression regulation as well as an evaluation of specific antibodies. ADAMTS-7 deficiency did not impair cell migration under proatherogenic conditions. ADAMTS7 expression was reduced by oxidized Low-density lipoprotein, which was partly reversible by Oxidized low-density lipoprotein receptor 1 knockdown.
- WWW:
- https://mediatum.ub.tum.de/?id=1543434
- Date of submission:
- 12.06.2020
- Oral examination:
- 26.10.2020
- File size:
- 5275659 bytes
- Pages:
- 161
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20201026-1543434-1-3
- Last change:
- 20.11.2020
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