The developed biosensor is a versatile, semi-automated, miniaturized, single-use, field screening instrument, with a power-autonomy of 6-8 hours, and temperature controlled. The concept of the instrument was built on the fundamentals of both flow-injection and immunoanalysis, and the main target analytes are TNT, atrazine, diuron, and isoproturon. The development consisted in transferring a series of immunochemicals from the traditional ELISA (enzyme-linked immunosorbent assay) format on microtiter plate onto the new biosensor platform. This transfer also required certain immunoassay developments and optimizations on microtiter plate and on golden surfaces (structures). The golden structures were used both isolated from and integrated into the sensor chip.
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The developed biosensor is a versatile, semi-automated, miniaturized, single-use, field screening instrument, with a power-autonomy of 6-8 hours, and temperature controlled. The concept of the instrument was built on the fundamentals of both flow-injection and immunoanalysis, and the main target analytes are TNT, atrazine, diuron, and isoproturon. The development consisted in transferring a series of immunochemicals from the traditional ELISA (enzyme-linked immunosorbent assay) format on microti...
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