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Document type:
Zeitschriftenaufsatz 
Author(s):
Wang, Q.; Tolkach, A.; Kulozik, U. 
Title:
Quantitative assessment of thermal denaturation of bovine α-lactalbumin via low-intensity ultrasound, HPLC, and DSC 
Abstract:
The degree of irreversible aggregation and the aggregation velocity constant of alpha-lactalbumin (alpha-la) were determined by three methods based on different principles: low-intensity ultrasound as a novel method for this purpose, DSC, and HPLC. The denaturation process of alpha-la causes a decrease in the ultrasonic velocity due to the conformation change of alpha-la molecules. This decrease is a function of the concentration of native alpha-la in the sample. A linear correlation was found b...    »
 
Keywords:
Animals; Calorimetry, Differential Scanning; Cattle; Chromatography, High Pressure Liquid; Hot Temperature; Kinetics; Lactalbumin/chemistry; Protein denaturation; Ultrasonics 
Journal title:
Journal of Agricultural and Food Chemistry 
Year:
2006 
Journal volume:
54 
Journal issue:
18 
Pages contribution:
6501--6506 
Fulltext / DOI: