Estradiol-17β (E2) is the most potent naturally occurring estrogen acting as a signaling molecule via epigenetic mechanisms. Here, a combined approach of methylation-sensitive high-resolution melting and pyrosequencing was created providing an effectively verified basis for DNA methylation analyses. With this methodology, abundance of the most important mediator of estrogenic action, estrogen receptor alpha (ESR1), was linked to a single CpG-site-based methylation difference impairing transcription factor binding. This epigenetic mechanism was not found influenced by endogenous E2 concentrations in male developing piglets. In contrast, E2 oral exposure to sows caused modified postnatal progeny body composition suggesting an epigenetic programming effect. Thus, potential effects of E2 seem to be subject to the susceptibility of the underlying developmental epigenetic mechanisms.
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Estradiol-17β (E2) is the most potent naturally occurring estrogen acting as a signaling molecule via epigenetic mechanisms. Here, a combined approach of methylation-sensitive high-resolution melting and pyrosequencing was created providing an effectively verified basis for DNA methylation analyses. With this methodology, abundance of the most important mediator of estrogenic action, estrogen receptor alpha (ESR1), was linked to a single CpG-site-based methylation difference impairing transcript...
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