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- Original title:
- Genexpressionsanalyse von Komponenten des Urokinase-Systems im Sphäroid-Mikrotumormodell
- Translated title:
- Gene expression analysis of components of the urokinase plasminogen activator system in microtumor-like 3D spheroids
- Author:
- Kohl, Christina Elisabeth
- Year:
- 2018
- Document type:
- Dissertation
- Faculty/School:
- Fakultät für Medizin
- Advisor:
- Magdolen, Viktor (Prof. Dr.)
- Referee:
- Magdolen, Viktor (Prof. Dr.); Kiechle, Marion B. (Prof. Dr.); Bassermann, Florian (Prof. Dr.)
- Language:
- de
- Subject group:
- MED Medizin
- TUM classification:
- MED 560d
- Abstract:
- Dreidimensionale (3D) Sphäroide spiegeln aufgrund ihrer Zell-Zell-Kontakte tumorassoziierte Prozesse besser wider als 2D-Monolayerzellen. Mittels qPCR wurde die mRNA Expression verschiedener tumorrelevanter Faktoren (uPA, uPAR, PAI-1, KLK7, Rab31) in 3D versus 2D kultivierten Zellen verschiedener Tumorentitäten untersucht und in vielen Fällen eine differentielle Genexpression in den Mikrotumor-ähnlichen Sphäroiden beobachtet, was die Bedeutung von 3D-Sphäroiden in der Tumorforschung unterstreich... »
- Translated abstract:
- Due to their three-dimensional cell-cell interactions, spheroids better reflect tumor-associated processes than 2D monolayer cells. mRNA expression of several tumor-relevant factors (uPA, uPAR, PAI-1, KLK7, Rab31) was analyzed via qPCR analysis in 3D versus 2D cultivated cells of different tumor entities. In many cases, differential gene expression was observed in microtumor-like spheroids compared to 2D- cultivated cells. The results highlight the importance of 3D spheroids in tumor research.
- WWW:
- https://mediatum.ub.tum.de/?id=1391212
- Date of submission:
- 04.10.2017
- Oral examination:
- 04.07.2018
- File size:
- 1859121 bytes
- Pages:
- 126
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20180704-1391212-1-0
- Last change:
- 05.09.2018
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