Translated abstract:
The correction of soft tissue defects is a challenge in plastic surgery. By using tissue-engineering techniques, generated autologous fat grafts represent a promising solution.
In this study we created a standard for preadipocytes isolation and cultivation. Furthermore preadipocytes were seeded on 3D polyurethane scaffolds, which were cultivated in vitro afterwards. Therefore a variation of culture media, different periods of digestion and the use of a MiniMacsTM System were tested.