Gushing is the spontaneous over-foaming of bottled carbonated beverages, particularly of beer. Due to its negative economical and image-damaging consequences it is of great interest for the beverage industry. To clarify the molecular causes of malt-related gushing, five surface-active proteins from filamentous fungi and from barley were heterologously expressed in
Pichia pastoris. The proteins were isolated and their gushing-inducing activity was determined. The protein FcHyd5p was identified as a potential gushing-inducer, whereas AfpA, nsLTP1 and FcHyd3p could be excluded as gushing-inducing substances. Furthermore, AfpA and nsLTP1 were categorized as gushing-inhibiting substances.
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Gushing is the spontaneous over-foaming of bottled carbonated beverages, particularly of beer. Due to its negative economical and image-damaging consequences it is of great interest for the beverage industry. To clarify the molecular causes of malt-related gushing, five surface-active proteins from filamentous fungi and from barley were heterologously expressed in
Pichia pastoris. The proteins were isolated and their gushing-inducing activity was determined. The protein FcHyd5p was identified as...
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