Comparison of immunoassays for the selective measurement of human high-molecular weight adiponectin.

Liu, D; Schuster, T; Baumann, M; Roos, M; Sollinger, D; Lutz, J; Heemann, U; von Eynatten, M

doi:10.1373/clinchem.2008.112425

journal article

Dokumenttyp:: Comparative Study; Journal Article
Autor(en):: Liu, D; Schuster, T; Baumann, M; Roos, M; Sollinger, D; Lutz, J; Heemann, U; von Eynatten, M
Titel:: Comparison of immunoassays for the selective measurement of human high-molecular weight adiponectin.
Abstract:: BACKGROUND: Adiponectin is an adipocyte-derived hormone circulating in different multimer complexes. The high-molecular-weight (HMW) complex is likely the active form of this protein and has been recognized as a risk marker for type 2 diabetes and coronary artery disease (CAD). Because quantification of HMW adiponectin by Western blot analysis is time-consuming, novel ELISAs have been developed to simplify measurements in clinical research. However, these enzyme immunoassays have not been cross-validated in larger patient groups. We evaluated 2 individual ELISA systems by comparison to Western blotting for measurement of the distribution of HMW adiponectin in healthy individuals and patients with CAD and type 2 diabetes. METHODS: We measured HMW adiponectin in 204 individuals (83 CAD patients, 81 type 2 diabetes patients, and 40 healthy controls). Correlations, range of agreement, and imprecision of HMW concentrations obtained using 2 commercial ELISAs (#1, ALPCO Diagnostics; #2, Millipore) were evaluated by comparison with quantitative Western blotting. RESULT: Adiponectin results of the ELISAs were significantly correlated with those obtained by Western blotting (both r > 0.75, P < 0.001). Deming regression and Bland-Altman analyses indicated high agreement among the 3 immunoassays. The median difference between HMW adiponectin concentrations measured by ELISA and by Western blot was +0.4 mg/L for ELISA #1 and -0.4 mg/L for ELISA #2 with 95% of value differences <3 mg/L. CONCLUSIONS: Selective measurement of HMW adiponectin by ELISA is feasible; however, individual differences among immunoassays must be considered. The evaluated ELISAs exhibit analytical characteristics that allow their use as equivalent for Western blot analysis in larger clinical and epidemiological groups.
Zeitschriftentitel:: Clin Chem
Jahr:: 2009
Band / Volume:: 55
Heft / Issue:: 3
Seitenangaben Beitrag:: 568-72
Sprache:: eng
Volltext / DOI:: doi:10.1373/clinchem.2008.112425
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/19168560
Print-ISSN:: 0009-9147
TUM Einrichtung:: Fachgebiet Nephrologie (Prof. Heemann); Institut für Medizinische Statistik und Epidemiologie
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