Benutzer: Gast  Login
Dokumenttyp:
journal article 
Autor(en):
Liu, D; Schuster, T; Baumann, M; Roos, M; Sollinger, D; Lutz, J; Heemann, U; von Eynatten, M 
Titel:
Comparison of immunoassays for the selective measurement of human high-molecular weight adiponectin. 
Abstract:
BACKGROUND: Adiponectin is an adipocyte-derived hormone circulating in different multimer complexes. The high-molecular-weight (HMW) complex is likely the active form of this protein and has been recognized as a risk marker for type 2 diabetes and coronary artery disease (CAD). Because quantification of HMW adiponectin by Western blot analysis is time-consuming, novel ELISAs have been developed to simplify measurements in clinical research. However, these enzyme immunoassays have not been cross-validated in larger patient groups. We evaluated 2 individual ELISA systems by comparison to Western blotting for measurement of the distribution of HMW adiponectin in healthy individuals and patients with CAD and type 2 diabetes. METHODS: We measured HMW adiponectin in 204 individuals (83 CAD patients, 81 type 2 diabetes patients, and 40 healthy controls). Correlations, range of agreement, and imprecision of HMW concentrations obtained using 2 commercial ELISAs (#1, ALPCO Diagnostics; #2, Millipore) were evaluated bycomparison with quantitative Western blotting. RESULT: Adiponectin results of the ELISAs were significantly correlated with those obtained by Western blotting (both r> 0.75, P< 0.001). Deming regression and Bland-Altman analyses indicated high agreement among the 3 immunoassays. The median difference between HMW adiponectin concentrations measured by ELISA and by Western blot was +0.4 mg/L for ELISA #1 and -0.4 mg/L for ELISA #2 with 95% of value differences<3 mg/L. CONCLUSIONS: Selective measurement of HMW adiponectin by ELISA is feasible; however, individual differences among immunoassays must be considered. The evaluated ELISAs exhibit analytical characteristics that allow their use as equivalent for Western blot analysis in larger clinical and epidemiological groups. 
Zeitschriftentitel:
Clin Chem 
Jahr:
2009 
Band / Volume:
55 
Heft / Issue:
Seitenangaben Beitrag:
568-72 
Sprache:
eng 
Print-ISSN:
0009-9147 
TUM Einrichtung:
r Medizinische Statistik und Epidemiologie; r Nephrologie