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Document type:
journal article 
Author(s):
Kallifatidis, G; Beckermann, BM; Groth, A; Schubert, M; Apel, A; Khamidjanov, A; Ryschich, E; Wenger, T; Wagner, W; Diehlmann, A; Saffrich, R; Krause, U; Eckstein, V; Mattern, J; Chai, M; Schütz, G; Ho, AD; Gebhard, MM; Büchler, MW; Friess, H; Büchler, P; Herr, I 
Title:
Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer. 
Abstract:
Genetic modification of human bone marrow mesenchymal stem cells (MSC) is highly valuable for their exploitation in basic science and therapeutic applications, for example in cancer. We present here a new, fast and easy-to-use method to enrich a functional population of lentiviral (LV)-transduced MSC expressing enhanced green fluorescent protein (eGFP). We replaced the eGFP gene by a fusion gene of puromycin acetyltransferase and eGFP. Upon LV gene transfer and puromycin selection, we quickly ob...    »
 
Journal title abbreviation:
Cancer Gene Ther 
Year:
2008 
Journal volume:
15 
Journal issue:
Pages contribution:
231-40 
Language:
eng 
Print-ISSN:
0929-1903 
TUM Institution:
Chirurgiesche Klinik und Poliklinik