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Dokumenttyp:
journal article 
Autor(en):
Zamò, A; Bertolaso, A; Franceschetti, I; Weirich, G; Capelli, P; Pecori, S; Chilosi, M; Hoefler, H; Menestrina, F; Scarpa, A 
Titel:
Microfluidic Deletion/Insertion Analysis for Rapid Screening of KIT and PDGFRA Mutations in CD117-Positive Gastrointestinal Stromal Tumors: Diagnostic Applications and Report of a New KIT Mutation. 
Abstract:
Gastrointestinal stromal tumors (GISTs) frequently harbor mutations in the KIT and PDGFRA genes, the presence and type of which correlate with the response to the kinase inhibitor imatinib mesylate. Because most GIST mutations are deletions/insertions, we used a microfluidic apparatus to detect these size variations in polymerase chain reaction-amplified DNA. This approach, termed microfluidic deletion/insertion analysis (MIDIA), identified mutations in 30 of 50 DNA samples from paraffin-embedde...    »
 
Zeitschriftentitel:
J Mol Diagn 
Jahr:
2007 
Band / Volume:
Heft / Issue:
Seitenangaben Beitrag:
151-7 
Sprache:
eng 
Print-ISSN:
1525-1578 
TUM Einrichtung:
r Allgemeine Pathologie und pathologische Anatomie