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Document type:
journal article 
Author(s):
Zamò, A; Bertolaso, A; Franceschetti, I; Weirich, G; Capelli, P; Pecori, S; Chilosi, M; Hoefler, H; Menestrina, F; Scarpa, A 
Title:
Microfluidic Deletion/Insertion Analysis for Rapid Screening of KIT and PDGFRA Mutations in CD117-Positive Gastrointestinal Stromal Tumors: Diagnostic Applications and Report of a New KIT Mutation. 
Abstract:
Gastrointestinal stromal tumors (GISTs) frequently harbor mutations in the KIT and PDGFRA genes, the presence and type of which correlate with the response to the kinase inhibitor imatinib mesylate. Because most GIST mutations are deletions/insertions, we used a microfluidic apparatus to detect these size variations in polymerase chain reaction-amplified DNA. This approach, termed microfluidic deletion/insertion analysis (MIDIA), identified mutations in 30 of 50 DNA samples from paraffin-embedde...    »
 
Journal title abbreviation:
J Mol Diagn 
Year:
2007 
Journal volume:
Journal issue:
Pages contribution:
151-7 
Language:
eng 
Print-ISSN:
1525-1578 
TUM Institution:
Institut für Allgemeine Pathologie und pathologische Anatomie