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Document type:
journal article 
Author(s):
Oberlaender, M; Bruno, RM; Sakmann, B; Broser, PJ 
Title:
Transmitted light brightfield mosaic microscopy for three-dimensional tracing of single neuron morphology. 
Abstract:
A fundamental challenge in neuroscience is the determination of the three-dimensional (3D) morphology of neurons in the cortex. Here we describe a semiautomated method to trace single biocytin-filled neurons using a transmitted light brightfield microscope. The method includes 3D tracing of dendritic trees and axonal arbors from image stacks of serial 100-microm-thick tangential brain sections. Key functionalities include mosaic scanning and optical sectioning, high-resolution image restoration,...    »
 
Journal title abbreviation:
J Biomed Opt 
Year:
2007 
Journal volume:
12 
Journal issue:
Pages contribution:
064029 
Language:
eng 
Print-ISSN:
1083-3668 
TUM Institution:
r Neurowissenschaften