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- Document type:
- Journal Article; Research Support, Non-U.S. Gov't; Article
- Author(s):
- Heim, N; Garaschuk, O; Friedrich, MW; Mank, M; Milos, RI; Kovalchuk, Y; Konnerth, A; Griesbeck, O
- Title:
- Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
- Abstract:
- Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
- Journal title abbreviation:
- Nat Methods
- Year:
- 2007
- Journal volume:
- 4
- Journal issue:
- 2
- Pages contribution:
- 127-9
- Language:
- eng
- Fulltext / DOI:
- doi:10.1038/nmeth1009
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/17259991
- Print-ISSN:
- 1548-7091
- TUM Institution:
- Institut für Neurowissenschaften
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