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Document type:
journal article 
Author(s):
Heim, N; Garaschuk, O; Friedrich, MW; Mank, M; Milos, RI; Kovalchuk, Y; Konnerth, A; Griesbeck, O 
Title:
Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. 
Abstract:
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits. 
Journal title abbreviation:
Nat Methods 
Year:
2007 
Journal volume:
Journal issue:
Pages contribution:
127-9 
Language:
eng 
Print-ISSN:
1548-7091 
TUM Institution:
r Neurowissenschaften