Benutzer: Gast  Login
Dokumenttyp:
journal article 
Autor(en):
Vogt, S; Ueblacker, P; Geis, C; Wagner, B; Wexel, G; Tischer, T; Krüger, A; Plank, C; Anton, M; Martinek, V; Imhoff, AB; Gansbacher, B 
Titel:
Efficient and stable gene transfer of growth factors into chondrogenic cells and primary articular chondrocytes using a VSV.G pseudotyped retroviral vector. 
Abstract:
Since efficient transfer of foreign genes into primary articular chondrocytes (CC) is difficult, a VSV.G pseudotyped retroviral vector (Bullet) was developed for marker and growth factor gene transfer. Transduction efficiency was analysed by FACS. BMP2 production was determined by specific hBMP2-ELISA. BMP2 effect on cells regarding proteoglycan production was measured by alcian blue staining and dye quantification. Alkaline phosphatase activity was determined by enzymatic reaction with p-nitrop...    »
 
Zeitschriftentitel:
Biomaterials 
Jahr:
2008 
Band / Volume:
29 
Heft / Issue:
Seitenangaben Beitrag:
1242-9 
Sprache:
eng 
Print-ISSN:
0142-9612 
TUM Einrichtung:
die; dische Klinik und Poliklinik; r experimentelle Onkologie und Therapieforschung 
Format:
Text