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Document type:
journal article 
Author(s):
Mykhaylyk, O; Antequera, YS; Vlaskou, D; Plank, C 
Title:
Generation of magnetic nonviral gene transfer agents and magnetofection in vitro. 
Abstract:
This protocol details how to design and conduct experiments to deliver nucleic acids to adherent and suspension cell cultures in vitro by magnetic force-assisted transfection using self-assembled complexes of nucleic acids and cationic lipids or polymers (nonviral gene vectors), which are associated with magnetic (nano) particles. These magnetic complexes are sedimented onto the surface of the cells to be transfected within minutes by the application of a magnetic gradient field. As the diffusio...    »
 
Journal title abbreviation:
Nat Protoc 
Year:
2007 
Journal volume:
Journal issue:
10 
Pages contribution:
2391-411 
Language:
eng 
Print-ISSN:
1754-2189 
TUM Institution:
Institut für experimentelle Onkologie und Therapieforschung