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Dokumenttyp:
journal article 
Autor(en):
Ueblacker, P; Wagner, B; Vogt, S; Salzmann, G; Wexel, G; Krüger, A; Plank, C; Brill, T; Specht, K; Hennig, T; Schillinger, U; Imhoff, AB; Martinek, V; Gansbacher, B 
Titel:
In vivo analysis of retroviral gene transfer to chondrocytes within collagen scaffolds for the treatment of osteochondral defects. 
Abstract:
To examine a retroviral gene transfer to chondrocytes in vitro and in vivo in tissue-engineered cell-collagen constructs articular chondrocytes from rabbits and humans were isolated and transduced with VSV.G pseudotyped murine leukemia virus-derived retroviral vectors. Viral supernatants were generated by transient transfection of 293T cells using the pBullet retroviral vector carrying the nlslacZ gene, a Moloney murine leukemia virus gag/pol plasmid and a VSV.G coding plasmid. Transduction effi...    »
 
Zeitschriftentitel:
Biomaterials 
Jahr:
2007 
Band / Volume:
28 
Heft / Issue:
30 
Seitenangaben Beitrag:
4480-7 
Sprache:
eng 
Print-ISSN:
0142-9612 
TUM Einrichtung:
die; r experimentelle Onkologie und Therapieforschung