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Document type:
Zeitschriftenaufsatz 
Author(s):
Frey, S; Haslbeck, M; Hainzl, O; Buchner, J 
Title:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system 
Abstract:
Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged proteins is usually performed in mammalian cells. Here, we describe the cell-free expression of a mouse monoclonal antibody, MAK33, in a coupled transcription/translation system, based on an Escherichia coli lysate. Both the heavy and the light chain can be pro...    »
 
Journal title:
Biol.Chem. 
Journal title abbreviation:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system 
Year:
2008 
Journal volume:
389 
Journal issue:
Pages contribution:
37-45 
Language:
en 
TUM Institution:
Lehrstuhl für Biotechnologie 
Format:
Text 
Ingested:
16.04.2008 
Last change:
21.04.2008