Benutzer: Gast  Login
Dokumenttyp:
Zeitschriftenaufsatz 
Autor(en):
Frey, S; Haslbeck, M; Hainzl, O; Buchner, J 
Titel:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system 
Abstract:
Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged proteins is usually performed in mammalian cells. Here, we describe the cell-free expression of a mouse monoclonal antibody, MAK33, in a coupled transcription/translation system, based on an Escherichia coli lysate. Both the heavy and the light chain can be pro...    »
 
Zeitschriftentitel:
Biol.Chem. 
Zeitschriftentitel:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system 
Jahr:
2008 
Band / Volume:
389 
Heft / Issue:
Seitenangaben Beitrag:
37-45 
Sprache:
en 
TUM Einrichtung:
Lehrstuhl für Biotechnologie 
Format:
Text 
Eingabe:
16.04.2008 
Letzte Änderung:
21.04.2008