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Original title:
Störexperimente an photosynthetisch aktiven Organismen. 
Original subtitle:
Untersuchungen zu Primär- und Sekundärstoffbiosynthesen in komplexen Stoffwechselnetzwerken. 
Translated title:
Perturbation/relaxation experiments with photosynthetic active organisms. Analysis of primary and secondary biosynthetic pathways in metabolic networks. 
Translated subtitle:
Untersuchungen zu Primär- und Sekundärstoffbiosynthesen in komplexen Stoffwechselnetzwerken. 
Year:
2005 
Document type:
Dissertation 
Institution:
Fakultät für Chemie 
Advisor:
Bacher, Adelbert (Prof. Dr. Dr.) 
Referee:
Bacher, Adelbert (Prof. Dr. Dr.); Gierl, Alfons (Prof. Dr.) 
Format:
Text 
Language:
de 
Subject group:
CHE Chemie 
Keywords:
Biosynthese; Stoffwechselnetzwerke; NMR Spektroskopie; Terpenbiosynthese 
Translated keywords:
biosynthetic; metabolic networks; NMR spectroscopy; terpenoid pathways 
Controlled terms:
Isoprenoide Biosynthese Kohlenstoff-13 Isotopenmarkierung Kohlenstoff-13-NMR-Spektroskopie Nicotin 
TUM classification:
CHE 692d; CHE 697d; CHE 804d 
Abstract:
Die Biosynthesewege der isoprenoiden Verbindungen Phytol, Geranylgeraniol, cis-Abienol und Sterole, sowie des Alkaloids Nicotin wurden in Markierungsexperimenten (in vivo) in Chloroflexus aurantiacus, Catharanthus roseus und Nicotiana tabacum (Wildtyp, RPO und NDH Mutanten) mit 13C-markierten Vorstufen [U-13C2]Glycin, [U-13C6]D-Glucose, [1-13C1]D-Glucose und 13CO2 untersucht.C. aurantiacus nutzt für die Terpenbiosynthese ausschließlich den Mevalonatweg. Die Anteile der beiden Terpenbiosynthesewe...    »
 
Translated abstract:
The biosynthetic pathways of the terpenoids phytol, geranylgeraniol, cis-abienol, sterols, as well as the alkaloid nicotin were analysed by in vivo incorporation experiments in Chloroflexus aurantiacus, Catharanthus roseus and Nicotiana tabacum (wildtyp, RPO and NDH mutants) using [U-13C2]glycine, [U-13C6]glucose, [1-13C1]glucose and sup>13CO2 as precursors.C. aurantiacus uses the mevalonate pathway for terpenoid biosynthesis. NMR spectroscopy was used to define the ratio between the two different terpenoid biosynthetic pathways in C. roseus and N. tabacum.The amino acid biosynthesis in the mutants of N. tabacum was found to be different from the wildtyp. The labeling patterns of lysine from C. roseus and N. tabacum could not be explained by the known biosynthetic pathways.The stereochemical processes of deoxyxylulose phosphate reductoisomerase and of sepiapterin reductase were delineated with selectively 2H labeled NADPH. 
Publication :
Universitätsbibliothek der TU München 
Oral examination:
09.02.2005 
File size:
5548943 bytes 
Pages:
367 
Last change:
25.03.2008