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Original title:
NMR- spektroskopische Untersuchungen von Actin-bindenden Proteinen aus Dictyostelium discoideum 
Translated title:
NMR- spectroscopic investigation of Actin binding proteins from Dictyostelium discoideum 
Year:
2003 
Document type:
Dissertation 
Institution:
Fakultät für Chemie 
Advisor:
Holak, Tad A. (Dr.) 
Referee:
Huber, Robert (Prof. Dr. Dr. h.c.); Gemmecker, Gerd (Priv.-Doz. Dr.) 
Format:
Text 
Language:
de 
Subject group:
CHE Chemie 
Keywords:
adenylat cyclase- assoziiertes protein; CAP; NMR Zuordung; Dictyostelium discoideum; Dictyostelium Discoideum actin interagierend protein 1 (DAip1); Proteinreinigung; Cofilin; Strukturbestimmung; dreidimensionale Struktur; zweidimensionale Struktur; Bündel von sechs Helices; Röntgenstrukturanalyse 
Translated keywords:
adenylyl cyclase-associated protein; CAP; NMR assignments; Dictyostelium discoideum; Dictyostelium Discoideum actin interacting protein 1 (DAip1); Protein Purification; Cofilin; Structure Determination; three-dimensional structure; two-dimensional structure; bundle of six helices; X-ray crystallography 
Controlled terms:
Dictyostelium discoideum; Actin-bindende Proteine; NMR-Spektroskopie 
TUM classification:
CHE 806d; CHE 820d; CHE 244d 
Abstract:
Das Cyclase-assoziierte-Protein (CAP) und seine Homologen sind G-Actin-sequestrierende Proteine. Die strukturelle Charakterisierung des D. discoideum CAP-Proteins erfolgte im Rahmen dieser Arbeit mittels magnetischer Kernresonanzspektroskopie. Dabei lag der Schwerpunkt in der Bestimmung der dreidimensionalen Struktur der stabilen N-terminalen Teildomäne des CAP-Proteins mit Hilfe von Distanzgeometrie- und molekulardynamischen Algorithmen. Die ermittelte Struktur stellt ein Bündel von sechs alpha...    »
 
Translated abstract:
The Dictyostelium discoideum cyclase associated protein (CAP) and its homologues of the higher and lower eukaryotic organisms are G-actin sequestering proteins. Our structural characterization of D. discoideum CAP was carried out using NMR spectroscopical methods, with the final aim to determine the three-dimensional structure of the amino-terminal core domain of CAP. The CAP-N structure consists of a bundle of six anti-parallels alpha-helices. A model of the full length of CAP was also proposed...    »
 
Publication :
Universitätsbibliothek der TU München 
Oral examination:
13.06.2003 
File size:
9715414 bytes 
Pages:
144 
Last change:
12.06.2007