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Document type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't 
Author(s):
Bishop, Derron; Niki?, Ivana; Brinkoetter, Mary; Knecht, Sharmon; Potz, Stephanie; Kerschensteiner, Martin; Misgeld, Thomas 
Title:
Near-infrared branding efficiently correlates light and electron microscopy. 
Abstract:
The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy. 
Journal title abbreviation:
Nat Methods 
Year:
2011 
Journal volume:
Journal issue:
Pages contribution:
568-70 
Language:
eng 
Print-ISSN:
1548-7091 
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)