Bishop, Derron, D; Niki?, Ivana, I; Brinkoetter, Mary, M; Knecht, Sharmon, S; Potz, Stephanie, S; Kerschensteiner, Martin, M; Misgeld, Thomas, T
Near-infrared branding efficiently correlates light and electron microscopy.
The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.