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Document type:
Zeitschriftenaufsatz 
Author(s):
Yu, Peng; Petzoldt, Svenja; Wilhelm, Mathias; Zolg, Daniel Paul; Zheng, Runsheng; Sun, Xuefei; Liu, Xiaodong; Schneider, Günter; Huhmer, Andreas; Kuster, Bernhard 
Title:
Trimodal Mixed Mode Chromatography That Enables Efficient Offline Two-Dimensional Peptide Fractionation for Proteome Analysis 
Abstract:
Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduce sample complexity and dynamic range and to utilize mass spectrometer (MS) time efficiently, high chromatographic resolution of and good orthogonality between the two dimensions are needed. Ion exchange and high pH reversed phase chromatography are often used for this purpose. However, the former requires desalting to be MS-compatible, and the latter requires fraction pooling to create orthogonal...    »
 
Keywords:
BayBioMS; Protein identificationPeptide identificationPeptides and proteinsIon exchangeChromatography 
Journal title:
Analytical Chemistry 
Year:
2017 
Journal volume:
89 
Journal issue:
17 
Pages contribution:
8884-8891 
Publisher:
American Chemical Society (ACS) 
E-ISSN:
0003-27001520-6882 
Date of publication:
18.08.2017