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Dokumenttyp:
journal article 
Autor(en):
Anastasov, Nata?a; Höfig, Ines; Mall, Sabine; Krackhardt, Angela M; Thirion, Christian 
Titel:
Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G. 
Abstract:
Lentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. This optimized LV infection protocol includes a nontoxic poloxamer-based adjuvant combined with antibody-retargeted lentiviral particles. The novel poloxamer P338 demonstrates superior characteristics for enhancing lentiviral transduction over the best-in-class polybrene-assisted transduction. Poloxamer P338 exhibited dual benefits of low toxicity and high efficiency of lentiviral gene...    »
 
Zeitschriftentitel:
Methods Mol Biol 
Jahr:
2016 
Band / Volume:
1448 
Seitenangaben Beitrag:
49-61 
Sprache:
eng 
Print-ISSN:
1064-3745 
TUM Einrichtung:
III. Medizinische Klinik und Poliklinik