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Document type:
journal article 
Author(s):
Takemura, K; Wang, P; Vorberg, I; Surewicz, W; Priola, SA; Kanthasamy, A; Pottathil, R; Chen, SG; Sreevatsan, S 
Title:
DNA aptamers that bind to PrP(C) and not PrP(Sc) show sequence and structure specificity. 
Abstract:
DNA aptamers were selected against recombinant human (rhu) cellular prion protein (PrP(C)) 23-231 by systematic evolution of ligands via a systematic evolution of ligands by exponential (SELEX) enrichment procedure using lateral flow chromatography. The SELEX procedure was performed with an aptamer library consisting of a randomized 40-nucleotide core flanked by 28-mer primer-binding sites that, theoretically, represented approximately 10(24) distinct nucleic acid species. Sixty nanograms of rhu...    »
 
Journal title abbreviation:
Exp Biol Med (Maywood) 
Year:
2006 
Journal volume:
231 
Journal issue:
Pages contribution:
204-14 
Language:
eng 
Print-ISSN:
1535-3702 
TUM Institution:
Institut für Virologie