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Dokumenttyp:
journal article 
Autor(en):
Garaschuk, O; Milos, RI; Konnerth, A 
Titel:
Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. 
Abstract:
One of the challenges for modern neuroscience is to understand the rules of concerted neuronal function in vivo. This question can be addressed using noninvasive high-resolution imaging techniques like two-photon microscopy. This protocol describes a versatile approach for in vivo two-photon calcium imaging of neural networks, stained with membrane-permeant fluorescent-indicator dyes. It is based on a targeted pressure ejection of the dye into the tissue of interest and can be used for a large s...    »
 
Zeitschriftentitel:
Nat Protoc 
Jahr:
2006 
Band / Volume:
Heft / Issue:
Seitenangaben Beitrag:
380-6 
Sprache:
eng 
Print-ISSN:
1754-2189 
TUM Einrichtung:
r Neurowissenschaften