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Dokumenttyp:
journal article 
Autor(en):
Durand, GM; Marandi, N; Herberger, SD; Blum, R; Konnerth, A 
Titel:
Quantitative single-cell RT-PCR and Ca(2+) imaging in brain slices. 
Abstract:
We have established a quantitative reverse transcriptase-PCR (RT-PCR) approach for the analysis of RNA transcript levels in individual cells of living brain slices. Quantification is achieved by using rapid-cycle, real-time PCR protocols and high-resolution external cDNA standard curves for the gene of interest. The method consists of several procedures, including cell soma harvest, reverse transcription, and an optimized cDNA purification step, which allowed us to quantify transcripts in small...    »
 
Zeitschriftentitel:
Pflugers Arch 
Jahr:
2006 
Band / Volume:
451 
Heft / Issue:
Seitenangaben Beitrag:
716-726 
Sprache:
eng 
Print-ISSN:
0031-6768 
TUM Einrichtung:
r Neurowissenschaften