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Document type:
journal article 
Author(s):
Hagen, RM; Seegmüller, I; Navai, J; Kappstein, I; Lehn, N; Miethke, T 
Title:
Development of a real-time PCR assay for rapid identification of methicillin-resistant Staphylococcus aureus from clinical samples. 
Abstract:
A major drawback of mecA PCR to detect methicillin-resistant Staphylococcus aureus (MRSA) directly from patient materials is the high frequency of methicillin-resistant coagulase-negative staphylococci. Therefore, a reliable detection method for MRSA from clinical samples using real-time PCR was developed. The PCR assay targeting the integration site (orfX) of the staphylococcal cassette chromosome mec (SCCmec) was evaluated in MRSA SCCmec reference strains (n = 9), MRSA ST strains (n = 16) and...    »
 
Journal title abbreviation:
Int J Med Microbiol 
Year:
2005 
Journal volume:
295 
Journal issue:
Pages contribution:
77-86 
Language:
eng 
Print-ISSN:
1438-4221 
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene