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Document type:
journal article 
Author(s):
Albers, C; Illert, AL; Miething, C; Leischner, H; Thiede, M; Peschel, C; Duyster, J 
Title:
An RNAi-based system for loss-of-function analysis identifies Raf1 as a crucial mediator of BCR-ABL-driven leukemogenesis. 
Abstract:
Genetic loss-of-function studies in murine tumor models have been essential in the analysis of downstream mediators of oncogenic transformation. Unfortunately, these studies are frequently limited by the availability of genetically modified mouse strains. Here we describe a versatile method allowing the efficient expression of an oncogene and simultaneous knockdown of targets of interest (TOI) from a single retroviral vector. Both oncogene and TOI-specific miR30-based shRNA are under the control...    »
 
Journal title abbreviation:
Blood 
Year:
2011 
Journal volume:
118 
Journal issue:
Pages contribution:
2200-10 
Language:
eng 
Print-ISSN:
0006-4971 
TUM Institution:
III. Medizinische Klinik und Poliklinik