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Dokumenttyp:
journal article 
Autor(en):
Sarafoff, NI; Bieschke, J; Giese, A; Weber, P; Bertsch, U; Kretzschmar, HA 
Titel:
Automated PrPres amplification using indirect sonication. 
Abstract:
Prions, which mainly consist of the scrapie isoform of the prion protein (PrP(Sc)), induce the misfolding of the physiological prion protein (PrP(C)). The Protein Misfolding Cyclic Amplification (PMCA), a process consisting of sonication and incubation, is one of the few methods thought to model autocatalytic prion replication and generation of proteinase K (PK)-resistant PrP (PrPres) in vitro. Here we show for the first time that the amplification may be achieved through direct as well as indir...    »
 
Zeitschriftentitel:
J Biochem Biophys Methods 
Jahr:
2005 
Band / Volume:
63 
Heft / Issue:
Seitenangaben Beitrag:
213-21 
Sprache:
eng 
Print-ISSN:
0165-022X 
TUM Einrichtung:
I. Medizinische Klinik und Poliklinik