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Document type:
journal article 
Author(s):
Ungerer, M; Peluso, M; Gillitzer, A; Massberg, S; Heinzmann, U; Schulz, C; Münch, G; Gawaz, M 
Title:
Generation of functional culture-derived platelets from CD34+ progenitor cells to study transgenes in the platelet environment. 
Abstract:
The possibility of evaluating the function of transgenes in platelets requires the generation of platelets from nucleated progenitor cells in vitro. In this article, we provide effective culture conditions for generating functional culture-derived (CD) human and mouse platelets from CD34(+) progenitor cells that allow expression of any foreign protein of interest. We have evolved an effective cytokine cocktail (thrombopoietin, stem cell factor, interleukin [IL]-1beta, IL-6) that induces a high y...    »
 
Journal title abbreviation:
Circ Res 
Year:
2004 
Journal volume:
95 
Journal issue:
Pages contribution:
e36-44 
Language:
eng 
Print-ISSN:
0009-7330 
TUM Institution:
I. Medizinische Klinik und Poliklinik