Benutzer: Gast  Login
Dokumenttyp:
journal article 
Autor(en):
Lehle, K; Hoenicka, M; Jacobs, VR; Schmid, FX; Birnbaum, DE 
Titel:
Identification and reduction of cryoinjury in endothelial cells: a first step toward establishing a cell bank for vascular tissue engineering. 
Abstract:
We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth rates, anti-inflammatory, and anticoagulant functions. Independent of cooling rate and storage time, the r...    »
 
Zeitschriftentitel:
Tissue Eng 
Jahr:
2006 
Band / Volume:
12 
Heft / Issue:
12 
Seitenangaben Beitrag:
3439-47 
Sprache:
eng 
Print-ISSN:
1076-3279 
TUM Einrichtung:
Frauenklinik und Poliklinik