Benutzer: Gast  Login
Dokumenttyp:
journal article 
Autor(en):
Bishop, Derron; Nikic, Ivana; Kerschensteiner, Martin; Misgeld, Thomas 
Titel:
The use of a laser for correlating light and electron microscopic images in thick tissue specimens. 
Abstract:
Correlating images between light and electron microscopy is difficult especially in tissue specimens with a substantial z-dimension. To facilitate correlated light and electron microscopy (CLEM) in thick tissue specimens, we describe a basic method of using a femto-pulsed near-infrared laser to burn precise three-dimensional fiducial markers that circumscribe cells or regions of interest for easy identification between imaging methods. This rapid and reliable approach permits traditional fixatio...    »
 
Zeitschriftentitel:
Methods Cell Biol 
Jahr:
2014 
Band / Volume:
124 
Seitenangaben Beitrag:
323-37 
Sprache:
eng 
Print-ISSN:
0091-679X 
TUM Einrichtung:
Institut für Zellbiologie des Nervensystems