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Dokumenttyp:
Zeitschriftenaufsatz 
Autor(en):
Kaufmann, Christine M.; Grassmann, Johanna; Letzel, Thomas 
Titel:
HPLC method development for the online-coupling of chromatographic Perilla frutescens extract separation with xanthine oxidase enzymatic assay 
Abstract:
Abstract Enzyme-regulatory effects of compounds contained in complex mixtures can be unveiled by coupling a continuous-flow enzyme assay to a chromatographic separation. A temperature-elevated separation was developed and the performance was tested using Perilla frutescens plant extracts of various polarity (water, methanol, ethanol/water). Owning to the need of maintaining sufficient enzymatic activity, only low organic solvent concentrations can be added to the mobile phase. Hence, to broaden...    »
 
Stichworte:
Inhibition; AF 
Zeitschriftentitel:
Journal of Pharmaceutical and Biomedical Analysis 
Jahr:
2016 
Band / Volume:
124 
Seitenangaben Beitrag:
347 - 357 
Print-ISSN:
0731-7085