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Document type:
journal article 
Author(s):
Li, Shun; Flisikowska, Tatiana; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Saur, Dieter; Kind, Alexander; Wolf, Eckhard; Flisikowski, Krzysztof; Schnieke, Angelika 
Title:
Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus. 
Abstract:
We are extending the Cre/loxP site-specific recombination system to pigs, focussing on conditional and tissue-specific expression of oncogenic mutations to model human cancers. Identifying the location, pattern and extent of Cre recombination in vivo is an important aspect of this technology. Here we report pigs with a dual fluorochrome cassette under the control of the strong CAG promoter that switches expression after Cre-recombination, from membrane-targeted tandem dimer Tomato to membrane-ta...    »
 
Journal title abbreviation:
PLoS ONE 
Year:
2014 
Journal volume:
Journal issue:
Pages contribution:
e102455 
Language:
eng 
TUM Institution:
II. Medizinische Klinik und Poliklinik