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Document type:
Zeitschriftenaufsatz 
Author(s):
Hekmat, Dariusch; Maslak, Dominik; Freiherr von Roman, Matthias; Breitschwerdt, Peter; Ströhle, Christoph; Vogt, Alexander; Berensmeier, Sonja; Weuster-Botz, Dirk 
Title:
Non-chromatographic preparative purification of enhanced green fluorescent protei 
Abstract:
Recombinant enhanced green fluorescent protein (eGFP) is used as a marker in numerous applications in biomedical research and diagnostics. For these applications, the macromolecule needs to be provided in a highly purified form. The conventional purification process of eGFP usually consists of multiple subsequent preparative chromatography steps. Since this procedure is costly and time-consuming, an alternative chromatography-free purification process was investigated. This process was a combina...    »
 
Keywords:
Clarified homogenized fermentation broth; Enhanced green fluorescent protein; Preparative crystallization; Protein purification; Stirred-tank crystallizer 
Journal title:
Journal of Biotechnology 
Year:
2015 
Journal volume:
194 
Year / month:
2015-01 
Pages contribution:
84-90 
Covered by:
Scopus; Web of Science 
Reviewed:
ja 
Language:
en