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Dokumenttyp:
Zeitschriftenaufsatz 
Autor(en):
Fraga García, P.; Freiherr von Roman, M.; Reinlein, S.; Wolf, M.; Berensmeier, S. 
Titel:
Impact of Nanoparticle Aggregation on Protein Recovery through a Pentadentate Chelate Ligand on Magnetic Carriers 
Abstract:
The growing need for more efficient separation techniques still dominates downstream processing of biomolecules, thus encouraging the continuous development of advanced anomaterials. In this paper we present an improved process for recovering recombinant histidine tagged green fluorescent protein from an E. coli cell lysate. Superparamagnetic ore−shell nanocarriers are functionalized with a pentadentate chelate affinity ligand and then loaded with metal ions (Cu2+, Ni2+, or Zn2+). The separati...    »
 
Stichworte:
protein recovery; biomolecule purification; magnetic separation; pentadentate chelate ligand; aggregation; agglomeration; nanoparticles 
Zeitschriftentitel:
ACS Applied Materials & Interfaces 
Jahr:
2014 
Jahr / Monat:
2014-06 
Sprache:
en 
TUM Einrichtung:
Fachgebiet für Selektive Trenntechnik