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Dokumenttyp:
journal article 
Autor(en):
Malinowsky, K; Wolff, C; Schott, C; Becker, KF 
Titel:
Characterization of signalling pathways by reverse phase protein arrays. 
Abstract:
Reverse phase protein array (RPPA) is a very suitable technique to analyze large numbers of proteins in small samples like for example tumor biopsies. Beside their small size another major hindrance for the analysis of proteins from biopsies is the extraction of proteins from formalin-fixed and paraffin-embedded (FFPE) tissues. Here we describe a protocol, allowing quantitative extraction of large numbers of proteins from FFPE tissues and their subsequent analysis by RPPA. To elucidate the role...    »
 
Zeitschriftentitel:
Methods Mol Biol 
Jahr:
2013 
Band / Volume:
1049 
Seitenangaben Beitrag:
285-99 
Sprache:
eng 
Print-ISSN:
1064-3745 
TUM Einrichtung:
Institut für Allgemeine Pathologie und pathologische Anatomie