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Document type:
journal article 
Author(s):
Falkowska-Hansen, B; Kollar, J; Grüner, BM; Schanz, M; Boukamp, P; Siveke, J; Rethwilm, A; Kirschner, M 
Title:
An inducible Tet-Off-H2B-GFP lentiviral reporter vector for detection and in vivo isolation of label-retaining cells. 
Abstract:
Many regenerative cells are label-retaining cells (LRCs) due to their ability to keep a DNA label over a prolonged time. Until recently, isolation of vital LRCs was hampered due to the necessary use of fixation methods. To circumvent this, we generated a lentiviral-(HIV-1) based vector expressing a Tet-Off controlled histone 2B-GFP (Tet-Off-H2B-GFP) reporter gene for the detection and isolation of viable LRCs. In initial experiments, the vector was successfully used to infect 2- and 3-dimensiona...    »
 
Journal title abbreviation:
Exp Cell Res 
Year:
2010 
Journal volume:
316 
Journal issue:
11 
Pages contribution:
1885-95 
Language:
eng 
Print-ISSN:
0014-4827 
TUM Institution:
II. Medizinische Klinik und Poliklinik