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Document type:
journal article 
Author(s):
Dzien, Piotr; Tee, Sui-Seng; Kettunen, Mikko I; Lyons, Scott K; Larkin, Timothy J; Timm, Kerstin N; Hu, De-En; Wright, Alan; Rodrigues, Tiago B; Serrao, Eva M; Marco-Rius, Irene; Mannion, Elizabeth; D'Santos, Paula; Kennedy, Brett W C; Brindle, Kevin M 
Title:
(13) C magnetic resonance spectroscopy measurements with hyperpolarized [1-(13) C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo. 
Abstract:
Dissolution dynamic nuclear polarization can increase the sensitivity of the (13) C magnetic resonance spectroscopy experiment by at least four orders of magnitude and offers a novel approach to the development of MRI gene reporters based on enzymes that metabolize (13) C-labeled tracers. We describe here a gene reporter based on the enzyme pyruvate decarboxylase (EC 4.1.1.1), which catalyzes the decarboxylation of pyruvate to produce acetaldehyde and carbon dioxide.Pyruvate decarboxylase from Z...    »
 
Journal title abbreviation:
Magn Reson Med 
Year:
2016 
Journal volume:
76 
Journal issue:
Pages contribution:
391-401 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
0740-3194 
TUM Institution:
Nuklearmedizinische Klinik und Poliklinik