Different chemical and biological catalysts were screened for the biphasic esterification of aqueous solutions of succinic acid with 1-octanol. Among them, DBSA, Nation NR-50 and Novozym 435 were found to be the most attractive catalysts. The pH, the temperature and the catalyst concentration had high impacts on the reaction rates. The optimization of the reaction conditions with a single-variable approach for the chemical catalysts and a Response Surface Methodology for the enzyme allowed an increase of the rates by a factor 1.5 for DBSA, 2.3 for Nation NR-50 and 1.3 for Novozym 435. Real fermentation broths produced from recombinant Escherichia coli could be successfully esterified with conversions up to 93     «

Different chemical and biological catalysts were screened for the biphasic esterification of aqueous solutions of succinic acid with 1-octanol. Among them, DBSA, Nation NR-50 and Novozym 435 were found to be the most attractive catalysts. The pH, the temperature and the catalyst concentration had high impacts on the reaction rates. The optimization of the reaction conditions with a single-variable approach for the chemical catalysts and a Response Surface Methodology for the enzyme allowed an in...     »

ISI Document Delivery No.: 966VS Times Cited: 0 Cited Reference Count: 38 Delhomme, Clara Goh, Serena L. M. Kuehn, Fritz E. Weuster-Botz, Dirk Kuhn, Fritz/A-3113-2010 IGSSE; Bavarian Research Foundation This work was generously supported by the International Graduate School of Science and Engineering (IGSSE) at the Technische Universitat Munchen, particularly by an IGSSE Ph.D. grant for Dr. Clara Delhomme. Dr. Serena LM. Goh is grateful to the Bavarian Research Foundation for a Ph.D. grant. The assistance of Elona Hasanbelli was greatly appreciated. 0 Elsevier science bv Amsterdam     «

ISI Document Delivery No.: 966VS Times Cited: 0 Cited Reference Count: 38 Delhomme, Clara Goh, Serena L. M. Kuehn, Fritz E. Weuster-Botz, Dirk Kuhn, Fritz/A-3113-2010 IGSSE; Bavarian Research Foundation This work was generously supported by the International Graduate School of Science and Engineering (IGSSE) at the Technische Universitat Munchen, particularly by an IGSSE Ph.D. grant for Dr. Clara Delhomme. Dr. Serena LM. Goh is grateful to the Bavarian Research Foundation for a Ph.D. grant. The...     »