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- Original title:
- Substrate-free Rhomboid Protease Assays for the Discovery of Novel Inhibitors
- Translated title:
- Substratfreie Testmethoden für Rhomboidproteasen zur Entdeckung neuer Inhibitoren
- Author:
- Wolf, Eliane Viola
- Year:
- 2015
- Document type:
- Dissertation
- Faculty/School:
- Fakultät Wissenschaftszentrum Weihenstephan
- Advisor:
- Verhelst, Steven (Dr.)
- Referee:
- Verhelst, Steven (Dr.); Lichtenthaler, Stefan (Prof. Dr.); Antes, Iris (Prof. Dr.)
- Language:
- en
- Subject group:
- BIO Biowissenschaften; CHE Chemie
- Keywords:
- activity-based probes, inhibitors, intramembrane proteolysis, rhomboids
- Translated keywords:
- Aktivitätsbasierte Sonden, Inhibitoren, Intramembranproteolyse, Rhomboide
- TUM classification:
- CHE 762d
- Abstract:
- Rhomboid proteases are intramembrane serine proteases which catalyze the cleavage of substrates within the plane of the membrane. In this thesis, substrate-free assays were developed by making use of activity-based probes (ABPs). These assays enabled the discovery and application of novel rhomboid inhibitors and ABPs, also for rhomboids not suitable for conventional inhibitor screens.
- Translated abstract:
- Rhomboidproteasen sind intramembranäre Serinproteasen, die Substrate in der Membranebene spalten. In dieser Arbeit wurden substratfreie Testmethoden entwickelt, die sich aktivitätsbasierte Sonden (eng: activity-based probes, ABPs) zunutze machen. Diese Testmethoden ermöglichten die Entdeckung und Anwendung neuer Rhomboidinhibitoren und ABPs, auch für Rhomboide, die nicht für konventionelle Inhibitorscreenings geeignet sind.
- WWW:
- https://mediatum.ub.tum.de/?id=1237864
- Date of submission:
- 28.01.2015
- Oral examination:
- 27.10.2015
- File size:
- 6996623 bytes
- Pages:
- 161
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20151027-1237864-1-9
- Last change:
- 10.12.2015
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