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Document type:
journal article 
Author(s):
Oberlaender, M; Dercksen, VJ; Egger, R; Gensel, M; Sakmann, B; Hege, HC 
Title:
Automated three-dimensional detection and counting of neuron somata. 
Abstract:
We present a novel approach for automated detection of neuron somata. A three-step processing pipeline is described on the example of confocal image stacks of NeuN-stained neurons from rat somato-sensory cortex. It results in a set of position landmarks, representing the midpoints of all neuron somata. In the first step, foreground and background pixels are identified, resulting in a binary image. It is based on local thresholding and compensates for imaging and staining artifacts. Once this pre...    »
 
Journal title abbreviation:
J Neurosci Methods 
Year:
2009 
Journal volume:
180 
Journal issue:
Pages contribution:
147-60 
Language:
eng 
Print-ISSN:
0165-0270 
TUM Institution:
Institut für Neurowissenschaften