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- Document type:
- Journal Article
- Author(s):
- Lahm, Harald; Singh, Niraj K; Gottmann, Irina; Doppler, Stefanie A; Dzilic, Elda; Preisler, Heike; Schneider, Stephanie; Lange, Rüdiger; Krane, Markus; Dreßen, Martina
- Title:
- Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technology.
- Abstract:
- The identification of TBX5-related regulatory sequences in genes essential for heart development is hampered by the absence of antibodies which allow precipitation of TBX5:DNA complexes. Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection... »
- Journal title abbreviation:
- Stem Cell Res
- Year:
- 2024
- Journal volume:
- 74
- Fulltext / DOI:
- doi:10.1016/j.scr.2023.103261
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/38100916
- Print-ISSN:
- 1873-5061
- TUM Institution:
- Klinik für Herz- und Gefäßchirurgie (DHM) (Prof. Krane)
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