Application of Raman Microscopy For a Rapid Antibiotic Susceptibility Test and the Investigation of Dormant Mycobacteria

Bauer, David

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Original title:: Application of Raman Microscopy For a Rapid Antibiotic Susceptibility Test and the Investigation of Dormant Mycobacteria
Translated title:: Anwendung der Raman Mikroskopie für einen schnellen Antibiotika Test und die Untersuchung des Dormanten Zustands von Mykobakterien
Author:: Bauer, David
Year:: 2021
Document type:: Dissertation
Faculty/School:: Fakultät für Chemie
Advisor:: Haisch, Christoph (Prof. Dr.)
Referee:: Haisch, Christoph (Prof. Dr.); Frieß, Wolfgang (Prof. Dr.)
Language:: en
Subject group:: CHE Chemie
Keywords:: Raman spectroscopy, deuterium, antibiotic susceptibility, heteroresistant bacteria, mycobacterium, dormant state
Translated keywords:: Raman Spektroskopie, Antibiotika, Heteroresistenz, deuterium, dormant, Mykobakterium
TUM classification:: CHE 200
Abstract:: Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D₂O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spectra. «
Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D₂O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spec... »
Translated abstract:: Die Raman-Spektroskopie ist eine vielversprechende Methode zur Analyse von Bakterien. Sie liefert Informationen über die molekulare Struktur mikroskopischer Objekte. In dieser Arbeit wurde ein Protokoll zur Messung der Stoffwechselaktivität von Bakterien anhand der Aufnahme von Deuterium entwickelt. Somit war eine schnelle Antibiotika-Testung, besonders von heteroresistenten Bakterien möglich. Auch wurde der dormante Zustand von M. smegmatis mittels Clusteranalyse von Raman-Spektren bestimmt.
WWW:: https://mediatum.ub.tum.de/?id=1610839
Date of submission:: 17.05.2021
Oral examination:: 13.07.2021
File size:: 4819338 bytes
Pages:: 119
Urn (citeable URL):: https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20210713-1610839-1-6
Last change:: 17.08.2021
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