Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D
2O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spectra.
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Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D
2O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spec...
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