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Document type:
Journal Article 
Author(s):
Marinkovi?, Petar; Godinho, Leanne; Misgeld, Thomas 
Title:
Generation of Tissue Sections for Screening Thy1 Mouse Lines. 
Abstract:
New generations of Thy1-XFP transgenic mice (where XFP stands for any fluorescent protein) can now be readily generated, given the availability of core facilities or commercial providers of Thy1 pronuclear injections. Here, we provide a protocol for screening founder progeny. Transcardial perfusion is performed on 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Cryosections are generated, and Thy1-driven expression is detected...    »
 
Journal title abbreviation:
Cold Spring Harb Protoc 
Year:
2015 
Journal volume:
2015 
Journal issue:
Pages contribution:
pdb.prot087684 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
1940-3402 
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)