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Document type:
Zeitschriftenaufsatz 
Author(s):
L. Janoschek; M. Freiherr von Roman; S. Berensmeier 
Title:
Protein A Affinity Precipitation of Human Immunoglobulin G 
Abstract:
The potential of protein A affinity precipitation as an alternative method for traditional antibody purification techniques was investigated. Recombinant produced protein A from Staphylococcus aureus (SpA) was covalently linked to the pH-responsive copolymer Eudragit® S-100 and used for purification of human immunoglobulin G (hIgG). The Eudragit-SpA conjugate had a static binding capacity of 93.9 ± 2.8 mg hIgG per g conjugate and a dissociation constant of 787 ± 67 nM at 7 ± 1 °C. The antibody w...    »
 
Keywords:
Antibody purification; Eudragit; Affinity precipitation; protein A; Immunoglobulin G 
Journal title:
Journal of Chromatography B 
Year:
2014 
Covered by:
Web of Science 
Reviewed:
ja 
Language:
en