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Document type:
journal article 
Author(s):
Ferizi, Mehrije; Leonhardt, Carolin; Meggle, Christian; Aneja, Manish K; Rudolph, Carsten; Plank, Christian; Rädler, Joachim O 
Title:
Stability analysis of chemically modified mRNA using micropattern-based single-cell arrays. 
Abstract:
The measurement of mRNA turnover in living cells plays an important role in the search for stable mRNA constructs for RNA-based therapies. Here we show that automated time-lapse microscopy combined with micropatterned arrays allows for efficient high-throughput monitoring of fluorescent reporter protein expression at the single-cell level. The fluorescence time courses after mRNA transfection yield the distribution of individual mRNA expression and degradation rates within a population. We compa...    »
 
Journal title abbreviation:
Lab Chip 
Year:
2015 
Journal volume:
15 
Journal issue:
17 
Pages contribution:
3561-71 
Language:
eng 
Print-ISSN:
1473-0197 
TUM Institution:
Institut für experimentelle Onkologie und Therapieforschung